Development of a miniaturized glucose and lactate sensor array for wireless bioreactor monitoring

To advance the field of process analytical technologies, there is a need for cheap, wireless, miniaturized sensor. Such sensors could be dropped into a microreactor and continuously monitor key critical parameters so that processes can be optimized in real-time. Imec has been working on developing reliable miniaturized electro-chemical sensors and ultra-low-power wireless interface electronics. In this project, the student will be developing technology towards that vision. Two of the key critical parameters to monitor are glucose and lactate consumption/production. Imec has developed an enzyme-based chemistry process compatible with planar technology.

In this research project, the student will be responsible to further develop, test and optimize a miniature glucose sensor based on novel microelectrode arrays and extend the work to lactate detection. The work will involve the fabrication of the microelectrode arrays using imec’s clean-room facilities, the formulation of the redox sensing chemistry as well as the functionalization of electrochemical sensors using automated spotting tools and testing in model solutions and cell culture vessels. The student will eventually design and have manufactured a small printed circuit board with the sensors and will be able to connect their work to imec’s miniature wireless interface electronics to validate their work in real bioreactors.

While this multidisciplinary project focuses on addressing the needs for better real-time measurements in biomanufacturing, the technology solution(s) being considered finds applications across medical, environmental, and industrial diagnostics as well, therefore providing valuable experience applicable to other research areas.

Required background:

For this master’s thesis, imec is searching for a highly motivated student that is eager to expand and/or build their knowledge and practical experience in bio/analytical sciences, electrochemistry, enzyme biochemistry, surface chemistry and biosensors and bioelectronics. Previous practical exposure to any of the cited field is a plus but not strictly necessary.

The student will be working within the context of an existing project, together with an international team of researchers. Hence, the ability to communicate fluently in English (spoken and written) is highly recommended.

Given the topic's highly interdisciplinary nature, we strongly advise the student to discuss the project with the supervisors prior to their application.