PhD - Leuven | More than two weeks ago
Disruptive microfluidic technology for single cell analysis
Single-cell processing is crucial in modern medicine. A well-known example is the revolutionary CAR-T cell therapy in which a patient’s own cells are harvested, engineered and readministered as a drug for cancer therapy. The isolation and characterization of antibody-producing cells with high selectivity and sensitivity is another example in today’s biomanufacturing requiring the screening of vast libraries of cells. Clearly, a need exists for the development of an innovative high-throughput analysis technology that offers single-cell resolution.
Developments in the field of microfluidics can help to meet these cell screening challenges. The precise fluidic structures with integrated microsensors and actuators allow detecting and manipulating single cells. Especially droplet microfluidics, in which cells are encapsulated in aqueous droplets in inert carrier oil, has become the de facto standard for single-cell applications. This principle is used in several commercial platforms for single-cell analysis (e.g. 10x Genomics, Mission Bio). Presently, constrained by technological limitations, these platforms are restricted to relatively simple workflows including droplet generation and incubation. Extending these platforms with more sophisticated droplet manipulations, such as droplet sorting, merging, splitting, reagent injection, will be needed to provide an answer to current industrial needs.
This PhD project will explore concepts for high-throughput electrical droplet manipulation based on silicon technology. The primary focus will be an extremely high-speed droplet sorter. Going beyond the state of the art, we will leverage semiconductor technologies for advanced electrode design and signal application to allow massive droplet sorting in multiple fluidic channels. Driven by application needs, we will advance the platform development from sorting to other droplet manipulations such as droplet merging or reagent injection. Finally, the platform will be demonstrated using state-of-the-art single-cell analysis assays. This topic will be supervised and supported by a team of physicists, engineers and biologists at imec, in collaboration with single-cell analysis experts of KU Leuven.
Required background: Electrical engineering, physics, biomedical engineering
Type of work: 50% design, simulation and fabrication; 50% characterization and experimental validation
Supervisor: Liesbet Lagae
Daily advisor: Chengxun Liu, Maarten Fauvart
The reference code for this position is 2021-083. Mention this reference code on your application form.