Exploring 3D-μEIT platform for dynamic, label-free, and non-invasive 3D tracking of organoid development and disease progression

Three-dimensional micro-electrical impedance tomography (3D-μEIT) is an emerging bioimaging technology that offers transformative potential for real-time, non-invasive, and label-free monitoring of biological dynamics and disease phenotypes. Beyond capturing morphological changes, 3D-μEIT enables the characterization of variations in tissue properties – such as density (linked to cell proliferation, apoptosis, extracellular matrix remodeling, and fibrosis), conductivity (influenced by ionic composition, fluid osmolarity, and pH), and barrier integrity – within 3D tissue-engineered constructs, spheroids, and organoids. These changes are reflected in electrical impedance, providing a unique window into biological processes that extends beyond traditional cytoarchitectural imaging.

We are currently inviting applications for a PhD position in a cutting-edge, interdisciplinary project centered on 3D-μEIT. The project aims to harness a newly developed 3D-μEIT platform at imec to monitor organoid development and pathological progression in selected disease models. We seek a highly motivated candidate with a passion for interdisciplinary research and a strong foundation – or keen interest – in areas such as stem cell biology, differentiation protocols, organoid culture and characterization, and 3D bioimaging.

Additional qualifications include fundamental knowledge of signal and systems theory, and proficiency in MATLAB or Python for computational modeling of biological systems. The candidate will receive training in molecular biology techniques (e.g., qPCR, cell viability and proliferation assays, ELISA, western blotting), immunohistochemistry, and confocal microscopy. Support will be provided for system performance optimization and image-based data analysis, with encouragement to explore broader applications across diverse biological contexts.