CMOS-based spatially single-cell gene editing and monitoring

Leuven - Master projects
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Ongeveer een week geleden

Single-cell sensing and transfection with non-viral vectors could be a key enabler of scalable techniques for cell therapy manufacturing, one of the hottest focus areas in pharma. In this project, A CMOS-based multielectrode array will be used to selectively monitor and electroporate cells deposited atop its surface to deliver gene-editing molecules.

This project is centred around a CMOS chip design featuring densely spaced, individually addressable, subcellular-sized electrodes for cell electrical analysis and for the delivery of molecules in adherent cell lines using electrical pulses (electroporation). A wide set of electrical pulse parameters will be screened to optimize cell survival and the efficiency of delivery of fluorescent molecules as measured by confocal microscopy combined with image processing. After successful delivery of plasmids into cells, the project will focus on the delivery of gene-editing molecules.


Type of project: Thesis

Required degree: Master of Engineering Technology, Master of Science, Master of Engineering Science, Master of Bioengineering

Required background: Biomedical engineering, Bioscience Engineering, Nanoscience & Nanotechnology

Supervising scientist(s): For further information or for application, please contact: Bastien Duckert (Bastien.Duckert@imec.be)

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