DNA Nanostructures for Active Nanopore Platforms: From Sensing to Molecular Control

Nanopore technology has revolutionized single-molecule sensing, enabling applications in genomics, proteomics, and diagnostics. Recent advances have introduced hybrid nanopore systems that combine biological and solid-state components. These innovations at the bio-nano interface offer new opportunities for enhanced analysis and synthesis functionality at the nanoscale.

This PhD project builds upon the work of Juliette Gevers, who developed a ring-shaped DNA origami nanostructure that scaffolds a lipid bicelle capable of hosting a biological nanopore. The goal of this follow-up PhD is to evolve the concept from a nanopore-as-a-sensor to a nanofactory-on-a-pore, where the nanopore becomes a dynamic nexus for monitoring and controlling molecular processes.

Key research directions include investigating the electrophoretic docking mechanisms of DNA nanostructures on solid-state nanopores, aiming to deepen our understanding of their interactions and stability. The project will also explore alternative strategies for nanostructure self-assembly and fixation, including both covalent and non-covalent approaches. Finally, the programmable nanoscale architecture of DNA origami will be harnessed to precisely position biochemical moieties, enabling applications such as targeted molecular analysis and controlled molecular synthesis.

This interdisciplinary topic combines nanotechnology, biophysics, and molecular engineering. The candidate will engage in both experimental and modeling work, contributing to the development of next-generation biosensing and molecular control platforms.