Study on Cryo-EM Support Grids for Enhanced Imaging Performance

Cryo-electron microscopy (cryo-EM) relies on grids that often suffer from uneven ice thickness and beam-induced motion, limiting resolution. This is especially problematic in the analysis of biomolecules and sub-cellular structures, where exposure has to be minimized, limiting the amount of information that can be gathered per grid. Hence, the search of more effective grids that offer better control on sample distribution and can be smoothly integrated in the preparation workflow is critical to the advance of the technique.  

This project proposes the design and nanofabrication of optimized cryo-EM grids using electron-beam lithography and thin-film deposition to improve mechanical stability and sample support. Finite Element Analysis (FEA) will guide grid geometry by simulating stress distribution and thermal contraction under cryogenic conditions, while parametric studies will optimize hole size, pitch, and support thickness for minimal deformation. 

The student will fabricate prototypes in imec experimental clean room, that will be characterized via SEM and AFM, followed by cryo-EM imaging to assess ice uniformity and particle distribution. The expected outcome, to be published in scientific journals, is a validated workflow combining simulation-driven design and nanofabrication to produce next-generation grids enabling higher-resolution structural studies.

Type of Internship:    PhD internship

Required educational background: Master of Engineering Technology; Master of Science; Master of Engineering Science; Master of Bioengineering

Duration:  1 year

For more information or application, please contact the supervising scientists Camila Dalben Campos (camila.campos@imec.be) and Ying Ting Set (ying.ting.set@imec.be).